Endobacter medicaginis gen. nov., sp. nov., isolated from alfalfa nodules in an acidic soil

• DOI: 10.1099/ijs.0.041368-0
• Publication Date: 2012-09-22T03:47:42Z
• Authors: Martha Helena Ram...; Carmen Tejedor; Isidro Martín; Encarna Velázquez; Alvaro Peix
• Keywords: Soil microbiology

A bacterial strain designated M1MS02 T was isolated from a surface-sterilized nodule of Medicago sativa in Zamora (Spain). The 16S rRNA gene sequence this showed 96.5 and 96.2 % similarity, respectively, with respect to Gluconacetobacter liquefaciens IFO 12388 Granulibacter bethesdensis CGDNIH1 the family Acetobacteraceae . novel isolate Gram-stain-negative, non-sporulating, aerobic coccoid rod-shaped bacterium that motile by subpolar flagellum. major fatty acid C 18 : 1 ω7 c ubiquinone Q-10. lipid profile consisted diphosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, three aminolipids, four glycolipids, phospholipids one lipid. Strain catalase-positive oxidase- urease-negative. Acetate lactate were not oxidized. Acetic produced ethanol culture media supplemented 2 CaCO 3 Ammonium sulphate assimilated glucose medium. dihydroxyacetone glycerol. Phylogenetic phenotypic analyses commonly used differentiate genera within should be classified as representing species new genus family, for which name Endobacter medicaginis gen. nov., sp. nov. is proposed. type ( = LMG 26838 CECT 8088 ). To our knowledge, first report member occurring legume endophyte.