Oscillibacter ruminantium sp. nov., isolated from the rumen of Korean native cattle

• DOI: 10.1099/ijs.0.041749-0
• Publication Date: 2012-10-01T02:09:22Z
• Authors: Geun-Hye Lee; Moon-Soo Rhee; Dong-Ho Chang; Jonghwan Lee; Seil Kim; Min Ho Yoon; Byoung-Chan Kim
• Keywords: Korean Native

A strictly anaerobic, Gram-negative, non-spore-forming bacterium, designated GH1(T), was isolated from the rumen of Korean native cattle (HanWoo). Cells were straight to slightly curved rods (2.0-4.5 µm long) and motile by peritrichous flagella. The isolate grew at 30-45 °C (optimum 40 °C), pH 5.5-6.5 6.0) with up 3.5% (w/v) NaCl. Strain GH1(T) produced acid d-glucose, d-ribose d-xylose, butyric being major end product. genomic DNA G+C content 54.6 mol%. Based on comparative 16S rRNA gene sequence analysis, strain most closely related Oscillibacter valericigenes Sjm18-20(T) (97.3% similarity). DNA-DNA hybridization between O. DSM 18026(T) showed 24% reassociation. fatty acids iso-C13:0 (13.0%), iso-C15:0 (17.6%), anteiso-C15:0 (8.4%) C14:0 (4.1%), cellular methyl esters as dimethylacetals (DMAs) C16:0 DMA (17.8%), (15.2%) (4.52%). cell-wall peptidoglycan contained meso-diaminopimelic sugar galactose. similarity, phylogenetic content, relatedness distinct phenotypic characteristics, is classified in genus a member novel species, for which name ruminantium sp. nov. proposed. type (=KCTC 15176(T)=NBRC 108824(T)=JCM 18333(T)).