Sinomicrobium oceani gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from marine sediment

• DOI: 10.1099/ijs.0.041889-0
• Publication Date: 2012-06-17T21:34:07Z
• Authors: Ying Xu; Xin-Peng Tian; Yu-Juan Liu; Jie Li; Chang-Jin Kim; Hao Yin; Wen-Jun Li; Si Zhang
• Keywords: DNA, Bacterial; 0301 basic medicine; Base Composition; China; Geologic Sediments; 0303 health sciences; Fatty Acids; Molecular Sequence Data; Vitamin K 2; Sequence Analysis, DNA; Bacterial Typing Techniques; 03 medical and health sciences; RNA, Ribosomal, 16S; Seawater; 14. Life underwater; Flavobacteriaceae; Phospholipids; Phylogeny

A marine bacterium, designated SCSIO 03483T, was isolated from a marine sediment sample collected from the Nansha Islands in the South China Sea. The strain produced roundish colonies with diffusible yellow-coloured pigment on nutrient agar medium or marine agar 2216. Optimal growth occurred in the presence of 0–4 % (w/v) NaCl, at pH 7.0 and a temperature range of 28–37 °C. 16S rRNA gene sequence analysis indicated that the isolate belonged to the familyFlavobacteriaceaeand showed relatively high sequence similarity withImtechella halotoleransK1T(92.7 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the isolate shared a lineage with members of the generaImtechella,JoostellaandZhouia. Phospholipids were phosphatidylethanolamine, two unidentified aminolipids and three unknown polar lipids. The major respiratory quinone was MK-6 and the major fatty acids were iso-C15 : 0, iso-C17 : 03-OH and summed feature 3 (C16 : 1ω6c/C16 : 1ω7c). The DNA G+C content of strain SCSIO 03483Twas 38.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain SCSIO 03483Trepresents a novel species in a new genus in the familyFlavobacteriaceae, for which the nameSinomicrobium oceanigen. nov., sp. nov. is proposed. The type strain ofSinobacterium oceaniis SCSIO 03483T( = KCTC 23994T = CGMCC 1.12145T).