Aliifodinibius roseus gen. nov., sp. nov., and Aliifodinibius sediminis sp. nov., two moderately halophilic bacteria isolated from salt mine samples

• DOI: 10.1099/ijs.0.043869-0
• Publication Date: 2013-01-19T05:39:57Z
• Authors: Yong-Xia Wang; Ji-Hui Liu; Wei Xiao; Xiao-Liang Ma; Yong-Hong Lai; Zhi-Ying Li; Kai-Yan Ji; Meng-Liang Wen; Xiao-Long Cui
• Keywords: DNA, Bacterial; 0301 basic medicine; China; Geologic Sediments; Bacteroidetes; Fatty Acids; Molecular Sequence Data; Nucleic Acid Hybridization; Vitamin K 2; Sequence Analysis, DNA; Sodium Chloride; Mining; 6. Clean water; Bacterial Typing Techniques; 3. Good health; 03 medical and health sciences; RNA, Ribosomal, 16S; Phylogeny

Two rod-shaped, non-motile bacteria were isolated from two separate salt mines in Yunnan, south-western China. These strains, designated YIM D15(T) and J21(T), Gram-negative moderately halophilic. The strains required 6-10 % NaCl (w/v; optimal) for growth. DNA G+C contents of J21(T) 49.0 mol% 48.4 mol%, respectively. predominant isoprenoid quinone was MK-7. polar lipid profiles composed predominantly diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, three unknown lipids one glycolipid. Minor amounts other also detectable. cellular fatty acids iso-C15 : 0, anteiso-C15 iso-C17 1ω9c/10 methyl-C16 0 C16 1ω7c/C16 1ω6c. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the isolates formed a distinct clade with genus Fodinibius (in phylum Bacteroidetes) related to species salinus, similarities 91.9-92.4 %. Analyses sequences revealed each (97.3 similarity). DNA-DNA hybridization relatedness between 34 On basis phylogenetic analysis, relatedness, phenotypic chemotaxonomic characteristics, should be classified as members novel species, which names Aliifodinibius roseus gen. nov., sp. nov. (type strain = ACCC 10715(T) KCTC 23442(T)) sediminis 10714(T) DSM 21194(T)) are proposed.