Salinisphaera japonica sp. nov., a moderately halophilic bacterium isolated from the surface of a deep-sea fish, Malacocottus gibber, and emended description of the genus Salinisphaera

• DOI: 10.1099/ijs.0.047845-0
• Publication Date: 2012-11-03T05:32:44Z
• Authors: Yasuhiro Shimane; Yusuke Tsuruwaka; Masayuki Miyazaki; Kozue Mori; Hiroaki Minegishi; Akinobu Echigo; Yukari Ohta; Tadashi Maruyama; William D. Grant; Yuji Hatada
• Keywords: DNA, Bacterial; Base Composition; 0303 health sciences; Ubiquinone; Fatty Acids; Molecular Sequence Data; Fishes; Sequence Analysis, DNA; 6. Clean water; Bacterial Typing Techniques; 03 medical and health sciences; RNA, Ribosomal, 16S; Animals; 14. Life underwater; Gammaproteobacteria; Phospholipids; Phylogeny

A moderately halophilic, slightly acidophilic, aerobic bacterium, designated strain YTM-1T, was isolated from the body surface of Malacocottus gibber. Cells were Gram-stain-negative, short rods or cocci, approximately 0.9–1.1 µm long and 1.0–1.8 µm wide. Strain YTM-1T was able to grow with 1–30 % NaCl (optimum, 7.5–10 %, w/v), at 4–30 °C (optimum, 20–25 °C) and at pH 3.8–9.5 (optimum, pH 5.0–5.5). Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strain YTM-1T belonged to the genus

Salinisphaera

with low similarity values to the type strains of recognized species of this genus (<94.8–94.4 %). The polar lipids of strain YTM-1T consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine, three unknown phospholipids and one unknown lipid. The predominant isoprenoid quinone was Q-8. The major fatty acids were C19 : 0ω8c cyclo, C18 : 1ω7c, C16 : 1ω5c and C16 : 0. The DNA G+C content of strain YTM-1T was 67.3 mol%. These phylogenetic, physiological and chemotaxonomic data indicated that strain YTM-1T represents a novel species of the genus

Salinisphaera

, for which the name Salinisphaera japonica sp. nov. is proposed. The type strain is YTM-1T ( = JCM 18087T = CECT 8012T). An emended description of the genus Salinisphaera is also proposed.