Streptococcus rubneri sp. nov., isolated from the human throat

• DOI: 10.1099/ijs.0.048538-0
• Publication Date: 2013-06-08T01:46:03Z
• Authors: Melanie Huch; Katrien De Bruyne; Ilse Cleenwerck; Achim Bub; Gyu-Sung Cho; Bernhard Watzl; Isabel Snauwaert; Charles M. A. P. ...; Peter Vandamme
• Keywords: ddc:796; DNA, Bacterial; Base Composition; 0303 health sciences; Molecular Sequence Data; Nucleic Acid Hybridization; Streptococcus; Sequence Analysis, DNA; DNA Fingerprinting; 796; Electrophoresis, Gel, Pulsed-Field; 3. Good health; 03 medical and health sciences; Athletic & outdoor sports & games; Genes, Bacterial; RNA, Ribosomal, 16S; Humans; Pharynx; Amplified Fragment Length Polymorphism Analysis; info:eu-repo/classification/ddc/796; Phylogeny

The novel, Gram-stain-positive, ovoid, lactic acid bacterial isolates LMG 27205, LMG 27206, LMG 27207Tand MRI-F 18 were obtained from throat samples of healthy humans. 16S rRNA gene sequence analyses indicated that these isolates belong to the genusStreptococcus, specifically theStreptococcus mitisgroup, withStreptococcus australisandStreptococcus mitisas the nearest neighbours (99.45 and 98.56 % 16S rRNA gene sequence similarity to the respective type strains). Genotypic fingerprinting by fluorescent amplified fragment length polymorphism (FAFLP) and pulsed-field gel electrophoresis (PFGE), DNA–DNA hybridizations, comparative sequence analysis ofpheS,rpoAandatpAand physiological and biochemical tests revealed that these bacteria formed a taxon well separated from its nearest neighbours and other species of the genusStreptococcuswith validly published names and, therefore, represent a novel species, for which the nameStreptococcus rubnerisp. nov. is proposed, with LMG 27207T( = DSM 26920T) as the type strain.