Halomicroarcula pellucida gen. nov., sp. nov., a non-pigmented, transparent-colony-forming, halophilic archaeon isolated from solar salt

• DOI: 10.1099/ijs.0.049965-0
• Publication Date: 2013-04-06T04:52:40Z
• Authors: Akinobu Echigo; Hiroaki Minegishi; Yasuhiro Shimane; Masahiro Kamekura; Takashi Itoh; Ron Usami
• Keywords: Phosphatidylglycerol; Yeast extract; Strain (injury); Nutrient agar

A novel halophilic strain, BNERC31(T), was isolated from solar salt, 'Sel marin de Guérande', imported France. Colonies on agar medium containing soluble starch, sodium citrate, glutamate and inorganic salts were non-pigmented transparent, while cells obtained by centrifuging liquid cultures red-pigmented. Cells of strain BNERC31(T) non-motile, pleomorphic, stained Gram-negative lysed in distilled water. Growth occurred with 20-30 % (w/v) NaCl (optimum, 25 %, w/v), 0-500 mM MgCl2 10 mM), at pH 6.0-8.5 7.0) 25-55 °C 40 °C). dependent inhibited completely 0.5 organic nutrients, such as Casamino acids or yeast extract. The DNA G+C content 64.1 mol%. Strain possessed least two heterogeneous 16S rRNA genes, the sequence orthologous gene (preceded dihydroorotate oxidase gene, pyrD) showed highest similarity (96.5 %) to that Haloarcula marismortui JCM 8966(T). RNA polymerase subunit B' (91.7 amylolytica 13557(T). polar lipids phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, sulfate, diglycosyl diether sulfated diether, similar those species genus Halomicrobium. phenotypic phylogenetic characteristics differed genera Halomicrobium indicated it represents a new genus, for which name Halomicroarcula pellucida gen. nov., sp. nov. is proposed. type ( = 17820(T) CECT 7537(T)).