Sphingomonas aerophila sp. nov. and Sphingomonas naasensis sp. nov., isolated from air and soil, respectively

• DOI: 10.1099/ijs.0.055269-0
• Publication Date: 2013-12-19T05:32:56Z
• Authors: Soo-Jin Kim; Ji-Young Moon; Jun-Muk Lim; Jae-Hyung Ahn; Hang-Yeon Weon; Tae-Young Ahn; Soon-Wo Kwon
• Keywords: DNA, Bacterial; 0303 health sciences; Spermidine; Ubiquinone; Fatty Acids; Molecular Sequence Data; Air Microbiology; Sequence Analysis, DNA; 15. Life on land; Sphingomonas; Bacterial Typing Techniques; 03 medical and health sciences; RNA, Ribosomal, 16S; Republic of Korea; Phospholipids; Phylogeny; Soil Microbiology

Two strains, designated 5413J-26Tand KIS18-15T, were isolated from the air and forest soil, respectively, in South Korea. Cells of the two strains were Gram-stain-negative, aerobic, polar-flagellated and rod-shaped. According to the phylogenetic tree, strains 5413J-26Tand KIS18-15Tfell into the cluster ofSphingomonassensu stricto. Strain 5413J-26Tshowed the highest sequence similarities withSphingomonas trueperiLMG 2142T(96.6 %),Sphingomonas molluscorumKMM 3882T(96.5 %),Sphingomonas azotifigensNBRC 15497T(96.3 %) andSphingomonas pituitosaEDIVT(96.1 %), while strain KIS18-15Thad the highest sequence similarity withSphingomonas soliT5-04T(96.8 %),Sphingomonas pituitosaEDIVT(96.6 %),Sphingomonas leidyiATCC 15260T(96.6 %),Sphingomonas asaccharolyticaNBRC 15499T(96.6 %) andSphingomonas koreensisJSS26T(96.6 %). The 16S rRNA gene sequence similarity between strains 5413J-26Tand KIS18-15Twas 95.4 %. Ubiquinone 10 was the predominant respiratory quinone and homospermidine was the major polyamine. The major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and several unidentified phospholipids and lipids. The main cellular fatty acids (>10 % of the total fatty acids) of strain 5413J-26Twere summed feature 8 (C18 : 1ω6cand/or C18 : 1ω7c), summed feature 3 (C16 : 1ω7cand/or iso-C15 : 02-OH) and C14 : 02-OH, and those of strain KIS18-15Twere summed feature 8 and C16 : 0. Based on the results of 16S rRNA gene sequence analysis, and physiological and biochemical characterization, two novel species with the suggested namesSphingomonas aerophilasp. nov. (type strain 5413J-26T = KACC 16533T = NBRC 108942T) andSphingomonas naasensissp. nov. (type strain KIS18-15T = KACC 16534T = NBRC 108943T) are proposed.