Thermoflexus hugenholtzii gen. nov., sp. nov., a thermophilic, microaerophilic, filamentous bacterium representing a novel class in the Chloroflexi, Thermoflexia classis nov., and description of Thermoflexaceae fam. nov. and Thermoflexales ord. nov.

• DOI: 10.1099/ijs.0.055855-0
• Publication Date: 2014-03-28T02:34:05Z
• Authors: Jeremy A. Dodsworth; Jonathan Gevorkian; Fairuz Despujos; Jessica K. Cole; Senthil K. Muruga...; Hong Ming; Wen-Jun Li; Gengxin Zhang; Alice Dohnalkova; Brian P. Hedlund
• Keywords: Chloroflexi (class); Microaerophile; Strain (injury); Yeast extract

A thermophilic, filamentous, heterotrophic bacterium, designated strain JAD2 T , a member of an as-yet uncultivated lineage that is present and sometimes abundant in some hot springs worldwide, was isolated from sediment Great Boiling Spring Nevada, USA. Cells had mean diameter 0.3 µm length 4.0 µm, formed filaments typically ranged 20 to 200 µm. Filaments were negative for the Gram stain reaction, spores not motility observed. The optimum temperature growth 72.5–75 °C, with range 67.5–75 pH 6.75, 6.5–7.75. Peptone, tryptone or yeast extract able support when supplemented vitamins, but no observed using variety defined organic substrates. Strain microaerophilic facultatively anaerobic, optimal at 1 % (v/v) O 2 upper limit 8 . major cellular fatty acids (>5 %) C 16 : 0 19 18 genomic DNA G+C content 69.3 mol%. Phylogenetic phylogenomic analyses sequences 16S rRNA gene other conserved genes placed within phylum Chloroflexi any existing class this phylum. These results indicate first cultivated representative novel which we propose name Thermoflexus hugenholtzii gen. nov., sp. Thermoflexia classis Thermoflexales ord. nov. Thermoflexaceae fam. type ( = JCM 19131 CCTCC AB-2014030 ).