Chryseomicrobium aureum sp. nov., a bacterium isolated from activated sludge

• DOI: 10.1099/ijs.0.061143-0
• Publication Date: 2014-05-15T08:57:00Z
• Authors: Shi-Kai Deng; Xiao-Mei Ye; Cui-Wei Chu; Jin Jiang; Jian He; Jun Zhang; Shun-Peng Li
• Keywords: DNA, Bacterial; 0301 basic medicine; China; 0303 health sciences; Sewage; Fatty Acids; Molecular Sequence Data; Nucleic Acid Hybridization; Vitamin K 2; Peptidoglycan; Sequence Analysis, DNA; 6. Clean water; Bacterial Typing Techniques; Waste Disposal Facilities; 03 medical and health sciences; Planococcaceae; RNA, Ribosomal, 16S; 11. Sustainability; Phospholipids; Phylogeny

A Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, aerobic bacterial strain, designated BUT-2T, was isolated from activated sludge of one herbicide-manufacturing wastewater-treatment facility in Kunshan, Jiangsu province, China, and subjected to polyphasic taxonomic studies. Analysis of the 16S rRNA gene sequence indicated that strain BUT-2Tshared the highest similarity withChryseomicrobium amylolyticum(98.98 %), followed byChryseomicrobium imtechense(98.88 %), with less than 96 % similarlity to members of the generaPaenisporosarcina,Planococcus,SporosarcinaandPlanomicrobium. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain BUT-2Tclustered withC. amylolyticumJC16TandC. imtechenseMW10T, occupying a distinct phylogenetic position. The major fatty acid (>10 % of total fatty acids) type of strain BUT-2Twas iso-C15 : 0.The quinone system comprised menaquinone MK-7 (77.8 %), MK-6 (11.9 %) and MK-8 (10.3 %). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and some unidentified phospholipids. The cell-wall peptidoglycan type of strain BUT-2Twasl-Orn-d-Glu. The genomic DNA G+C content of strain BUT-2Twas 48.5 mol%. Furthermore, the DNA–DNA relatedness in hybridization experiments against the reference strain was lower than 70 %, confirming that strain BUT-2Tdid not belong to previously described species of the genusChryseomicrobium. On the basis of its morphological, physiological and chemotaxonomic characteristics as well as phylogenetic analysis, strain BUT-2Tis considered to represent a novel species of the genusChryseomicrobium, for which the nameChryseomicrobiumaureumsp. nov. is proposed. The type strain is BUT-2T( = CCTCC AB2013082T = KACC 17219T).