Alicyclobacillus cellulosilyticus sp. nov., a thermophilic, cellulolytic bacterium isolated from steamed Japanese cedar chips from a lumbermill

• DOI: 10.1099/ijs.0.061440-0
• Publication Date: 2014-04-08T02:43:29Z
• Authors: Masataka Kusube; Asami Sugihara; Yoshito Moriwaki; Takahiro Ueoka; Yasuhiro Shimane; Hiroaki Minegishi
• Keywords: DNA, Bacterial; 0301 basic medicine; Alicyclobacillus; Cryptomeria; Fatty Acids; Molecular Sequence Data; Vitamin K 2; Sequence Analysis, DNA; Wood; 6. Clean water; Bacterial Typing Techniques; 03 medical and health sciences; Japan; RNA, Ribosomal, 16S; Phylogeny

A thermophilic bacterium, strain SueokaT, was isolated from steamed Japanese cedar chips from a lumber mill in Gobo, Japan. The strain was able to grow on carboxymethyl cellulose at 60 °C, was Gram-stain-negative, and grew between 40.0 and 67.5 °C (optimum at 55 °C) and between pH 3.5 and 6.5 (optimum at pH 4.8). Comparative analysis of 16S rRNA gene sequences revealed 91.9 , 90.9 , and 90.8 % similarity toAlicyclobacillus macrosporangiidusT,Alicyclobacillus pomorumT, andAlicyclobacillus acidocaldariusT, respectively. The major quinone was MK-7 and the predominant cellular fatty acids wereω-cyclohexane C19 : 0andω-cyclohexane C17 : 0. The DNA G+C content was 60.8 mol%. Based on the results of this study, strain SueokaTis a novel species of the genusAlicyclobacillus, and the namehttp://dx.doi.org/10.1601/nm.5071Alicyclobacilluscellulosilyticussp. nov. (type strain SueokaT = JCM 18487T = KCTC 33007T) is proposed.