Bacillus fengqiuensis sp. nov., isolated from a typical sandy loam soil under long-term fertilization

• DOI: 10.1099/ijs.0.063081-0
• Publication Date: 2014-05-29T02:50:47Z
• Authors: Fei Zhao; You-zhi Feng; Rui-rui Chen; Hua-yong Zhang; Jun-hua Wang; Xian-gui Lin
• Keywords: DNA, Bacterial; 0301 basic medicine; Base Composition; China; 0303 health sciences; Fatty Acids; Molecular Sequence Data; Nucleic Acid Hybridization; Bacillus; Vitamin K 2; Peptidoglycan; Sequence Analysis, DNA; Diaminopimelic Acid; 6. Clean water; 03 medical and health sciences; 13. Climate action; RNA, Ribosomal, 16S; Phylogeny; Soil Microbiology

A Gram-staining-positive, endospore-forming, moderately alkaliphilic bacterium, strain NPK15T, was isolated from a typical sandy loam soil under long-term NPK fertilization in northern China and was subjected to a polyphasic taxonomic study. The diamino acid of the cell-wall peptidoglycan of strain NPK15T was found to be meso-diaminopimelic acid and the cell-wall sugars were xylose, glucose and traces of mannose. The only respiratory quinone found in strain NPK15T was menaquinone 7 (MK-7). The major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and C16 : 1ω6c/C16 : 1ω7c. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analysis of the strain based on its 16S rRNA gene sequence showed that it was related most closely to ‘

Bacillus thaonhiensis

’ KACC 17216 (99.59 %),

B. songklensis

KCTC 13881T (99.52 %) and

B. abyssalis

CCTCC AB 2012074T (99.00 %). DNA–DNA hybridization results indicated that the strain was distinct from other species of the genus

Bacillus

, the degree of relatedness being 35.4 % with

B. abyssalis

CCTCC AB 2012074T, 39.7 % with

B. songklensis

KCTC 13881T and 51.2 % with ‘

B. thaonhiensis

’ KACC 17216. The DNA G+C content of strain NPK15T was 45.5 mol%. Phenotypic, chemotaxonomic and molecular analyses identified strain NPK15T as a member of a novel species of the genus

Bacillus

, for which the name Bacillus fengqiuensis sp. nov. is proposed. The type strain is NPK15T ( = DSM 26745T = CCTCC AB 2013156T).