Phenylobacterium kunshanense sp. nov., isolated from the sludge of a pesticide manufacturing factory

• DOI: 10.1099/ijs.0.063644-0
• Publication Date: 2014-10-29T06:18:49Z
• Authors: Cuiwei Chu; Cansheng Yuan; Xin Liu; Li Yao; Jianchun Zhu; Jian He; Soon-Wo Kwon; Xing Huang
• Keywords: DNA, Bacterial; 0301 basic medicine; Base Composition; China; Sewage; Ubiquinone; Fatty Acids; Molecular Sequence Data; Nucleic Acid Hybridization; Caulobacteraceae; Sequence Analysis, DNA; 6. Clean water; Bacterial Typing Techniques; 03 medical and health sciences; RNA, Ribosomal, 16S; Pesticides; Phylogeny

A novel aerobic, Gram-stain-negative, motile bacterium, designated strain BUT-10T, was isolated from the sludge of a pesticide manufacturing factory in Kunshan, China. Cells were rod-shaped (0.4–0.45×0.9–1.4 µm) and colonies were white, circular with entire edges and had a smooth surface. The strain grew at 25–37 °C, at pH 6.0–8.0 and with 0–0.5 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain BUT-10T was a member of the genus

Phenylobacterium

, and showed highest sequence similarities to

Phenylobacterium muchangponense

A8T (97.49 %),

Phenylobacterium immobile

DSM 1986T (97.14 %) and

Phenylobacterium lituiforme

FaiI3T (96.34 %). Major fatty acids (>5 %) were summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c), C16 : 0 and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c). The major isoprenoid quinone was ubiquinone-10. The DNA G+C content was 71.85 mol%. Strain BUT-10T showed low DNA–DNA relatedness with

P. muchangponense

A8T (15.7±2.9 %) and

P. immobile

DSM 1986T (12.8±1.1 %). On the basis of the phenotypic, phylogenetic and genotypic data, strain BUT-10T is considered to represent a novel species of the genus

Phenylobacterium

, for which the name Phenylobacterium kunshanense sp. nov. is proposed. The type strain is BUT-10T ( = CCTCC AB 2013085T = KCTC 42014T).