Dissection of the genus Actinobaculum: Reclassification of Actinobaculum schaalii Lawson et al. 1997 and Actinobaculum urinale Hall et al. 2003 as Actinotignum schaalii gen. nov., comb. nov. and Actinotignum urinale comb. nov., description of Actinotignum sanguinis sp. nov. and emended descriptions of the genus Actinobaculum and Actinobaculum suis; and re-examination of the culture deposited as Actinobaculum massiliense CCUG 47753T ( = DSM 19118T), revealing that it does not represent a strain of this species

• DOI: 10.1099/ijs.0.069294-0
• Publication Date: 2014-11-19T04:34:35Z
• Authors: A. F. Yassin; C. Spröer; R. Pukall; M. Sylvester; C. Siering; P. Schumann
• Keywords: DNA, Bacterial; Base Composition; 0303 health sciences; Fatty Acids; Molecular Sequence Data; Nucleic Acid Hybridization; Peptidoglycan; Sequence Analysis, DNA; Bacterial Typing Techniques; 3. Good health; 03 medical and health sciences; RNA, Ribosomal, 16S; Actinomycetaceae; Phylogeny

The remarkable host specificity of the species of the genusActinobaculumled us to recharacterize these species by a polyphasic approach. A comparative chemotaxonomic study including analysis of whole-cell sugars, amino acid composition of the peptidoglycan, fatty acid methyl esters, respiratory quinones and polar lipids revealed significant differences that, in combination with molecular data, support a dissection of the genusActinobaculum. The proposals of this study include the reclassification ofActinobaculum schaaliiandActinobaculum urinaleasActinotignum schaaliigen. nov., comb. nov. (type strain DSM 15541T = CCUG 27420T) andActinotignum urinalecomb. nov. (type strain DSM 15805T = CCUG 46093T), respectively. Emended descriptions of the genusActinobaculumandActinomyces suisare also provided. The results of 16S rRNA gene sequence analysis and DNA–DNA hybridization also indicated that the type strain ofActinobaculum massiliensedeposited as CCUG 47753T( = DSM 19118T) should in fact be considered a member of the speciesActinobaculum schaalii. In addition, comparative 16S rRNA gene sequencing and DNA–DNA relatedness studies of four strains recovered from clinical materials demonstrated that three of the isolates belonged toActinotignum schaalii; the remaining strain represents a novel species, for which the nameActinotignum sanguinissp. nov. is proposed. The type strain is IMMIB L-2199T( = DSM 26039T = CCUG 64068T).