Dissection of the genus Actinobaculum: Reclassification of Actinobaculum schaalii Lawson et al. 1997 and Actinobaculum urinale Hall et al. 2003 as Actinotignum schaalii gen. nov., comb. nov. and Actinotignum urinale comb. nov., description of Actinotignum sanguinis sp. nov. and emended descriptions of the genus Actinobaculum and Actinobaculum suis; and re-examination of the culture deposited as Actinobaculum massiliense CCUG 47753T ( = DSM 19118T), revealing that it does not represent a strain of this species

DNA, Bacterial 0301 basic medicine Base Composition 0303 health sciences Fatty Acids Molecular Sequence Data Nucleic Acid Hybridization Peptidoglycan Sequence Analysis, DNA Bacterial Typing Techniques 3. Good health 03 medical and health sciences RNA, Ribosomal, 16S Actinomycetaceae Phylogeny
DOI: 10.1099/ijs.0.069294-0 Publication Date: 2014-11-19T04:34:35Z
ABSTRACT
The remarkable host specificity of the species genus Actinobaculum led us to recharacterize these by a polyphasic approach. A comparative chemotaxonomic study including analysis whole-cell sugars, amino acid composition peptidoglycan, fatty methyl esters, respiratory quinones and polar lipids revealed significant differences that, in combination with molecular data, support dissection Actinobaculum. proposals this include reclassification schaalii urinale as Actinotignum gen. nov., comb. nov. (type strain DSM 15541(T) = CCUG 27420(T)) 15805(T) 46093(T)), respectively. Emended descriptions Actinomyces suis are also provided. results 16S rRNA gene sequence DNA-DNA hybridization indicated that type massiliense deposited 47753(T) ( 19118(T)) should fact be considered member schaalii. In addition, sequencing relatedness studies four strains recovered from clinical materials demonstrated three isolates belonged schaalii; remaining represents novel species, for which name sanguinis sp. is proposed. IMMIB L-2199(T) 26039(T) 64068(T)).
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