Haloterrigena hispanica sp. nov., an extremely halophilic archaeon from Fuente de Piedra, southern Spain

• DOI: 10.1099/ijs.0.64895-0
• Publication Date: 2007-07-11T23:20:08Z
• Authors: Ida Romano; Annarita Poli; Ilaria Finore; F. Javier Huertas; Agata Gambacorta; Salvatore Pellicc...; Giancarlo Nicolaus; Licia Lama; Barbara Nicolaus
• Keywords: 0301 basic medicine; Base Composition; Halobacteriaceae; Molecular Sequence Data; HALOMONAS-ANTICARIENSIS; Magnesium Chloride; Temperature; Nucleic Acid Hybridization; Genes, rRNA; RNA, Archaeal; Sequence Analysis, DNA; Sodium Chloride; DNA, Ribosomal; 03 medical and health sciences; DNA, Archaeal; Spain; RNA, Ribosomal, 16S; Sequence Homology, Nucleic Acid; SALT LAKE; GEN. NOV.; Glycolipids; Water Microbiology; Phylogeny

An extremely halophilic archaeon belonging to the order Halobacteriales was isolated from Fuente de Piedra salt lake, Spain. This strain, designated FP1 T , a pleomorphic coccoid, neutrophilic and required at least 15 % (w/v) NaCl for growth. Strain grew 37–60 °C, with optimal growth 50 °C. Mg 2+ not required, but observed up 10 MgSO 4 . Polar lipid analysis revealed presence of mannose-6-sulfate(1-2)-glucose glycerol diether as major glycolipid. Both C 20 25 core lipids were present. The genomic DNA G+C content 62.0 mol%. Phylogenetic based on comparison 16S rRNA gene sequences demonstrated that isolate most closely related species genus Haloterrigena DNA–DNA reassociation values between strain ( thermotolerans saccharevitans limicola ) lower than 29 %. It is therefore considered represents novel which name hispanica sp. nov. proposed. type (=DSM 18328 =ATCC BAA-1310 ).