Halorubrum arcis sp. nov., an extremely halophilic archaeon isolated from a saline lake on the Qinghai–Tibet Plateau, China

• DOI: 10.1099/ijs.0.64921-0
• Publication Date: 2007-05-01T20:03:01Z
• Authors: Xue-Wei Xu; Yue-Hong Wu; Hui-bin Zhang; Min Wu
• Keywords: Base Composition; China; 0303 health sciences; Halobacteriaceae; Nitrogen; Molecular Sequence Data; Nucleic Acid Hybridization; Genes, rRNA; Sequence Analysis, DNA; DNA, Ribosomal; Carbon; 03 medical and health sciences; DNA, Archaeal; RNA, Ribosomal, 16S; Sequence Homology, Nucleic Acid; Carbohydrate Metabolism; Phenazines; Gentian Violet; Water Microbiology; Phospholipids; Phylogeny

A Gram-negative, aerobic, neutrophilic and extremely halophilic archaeon (strain AJ201T), isolated from Ayakekum salt lake on the Qinghai–Tibet Plateau, was investigated by a polyphasic approach. The DNA G+C content of strain AJ201Twas 65.7 mol%. The major polar lipid profile and phylogenetic analysis based on 16S rRNA gene sequences supported the allocation of the strain to the genusHalorubrum. The results of DNA–DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain AJ201Tfrom closely related species. Therefore, strain AJ201Trepresents a novel species of the genusHalorubrum, for which the nameHalorubrum arcissp. nov. is proposed. The type strain is strain AJ201T(=CGMCC 1.5343T=JCM 13916T).