A multilocus phylogeny of the Streptomyces griseus 16S rRNA gene clade: use of multilocus sequence analysis for streptomycete systematics

• DOI: 10.1099/ijs.0.65224-0
• Publication Date: 2008-01-05T01:43:22Z
• Authors: Y. Guo; W. Zheng; X. Rong; Y. Huang
• Keywords: Streptomyces griseus; Housekeeping gene; Multilocus sequence typing

Streptomycetes are a complex group of actinomycetes that produce diverse bioactive metabolites commercial significance. Systematics can provide useful framework for identifying species may novel metabolites. However, previously proposed approaches to the systematics Streptomyces have suffered from either poor interlaboratory comparability or insufficient resolution. In particular, griseus 16S rRNA gene clade is most challenging and least defined within genus in terms phylogeny. Here we report results multilocus sequence analysis scheme developed address phylogeny this clade. Sequence fragments six housekeeping genes, atpD, gyrB, recA, rpoB, trpB rRNA, were obtained 53 reference strains represent 45 valid subspecies. Analysis each individual locus confirmed suitability loci congruence single-gene trees concatenation. Concatenated three, four, five all genes constructed, stability topology discriminatory power tree analysed. It be concluded phylogenetic based on sequences more accurate robust delineation Streptomyces. A proved optimal elucidating interspecies relationships S. Our provides valuable tool applied other clades refining systematic genus.