Brucella microti sp. nov., isolated from the common vole Microtus arvalis

• DOI: 10.1099/ijs.0.65356-0
• Publication Date: 2008-01-24T20:24:40Z
• Authors: H. C. Scholz; Z. Hubalek; I. Sedlacek; G. Vergnaud; H. Tomaso; S. Al Dahouk; F. Melzer; P. Kampfer; H. Neubauer; A. Cloeckaert; M. Maquart; M. S. Zygmunt; A. M. Whatmore; E. Falsen; P. Bahn; C. Gollner; M. Pfeffer; B. Huber; H.-J. Busse; K. Nockler
• Keywords: Multilocus sequence typing; Biovar; Ribotyping

Two Gram-negative, non-motile, non-spore-forming, coccoid bacteria (strains CCM 4915T and 4916), isolated from clinical specimens of the common vole Microtus arvalis during an epizootic in Czech Republic 2001, were subjected to a polyphasic taxonomic study. On basis 16S rRNA (rrs) recA gene sequence similarities, both isolates allocated genus Brucella. Affiliation Brucella was confirmed by DNA–DNA hybridization studies. Both strains reacted equally with M-monospecific antiserum lysed bacteriophages Tb, Wb, F1 F25. Biochemical profiling revealed high degree enzyme activity metabolic capabilities not observed other species. The omp2a omp2b genes 4916 indistinguishable. Whereas identical brucellae certain pinniped marine mammals, clustered terrestrial brucellae. Analysis bp26 downstream region identified as origin. harboured five six copies insertion element IS711, displaying unique banding pattern determined Southern blotting. In comparative multilocus VNTR (variable-number tandem-repeat) analysis (MLVA) 296 different genotypes, two grouped together, but formed separate cluster within Multilocus typing (MLST) using nine loci also placed separately IS711-based AMOS PCR, 1900 bp fragment generated ovis-specific primers, revealing that had integrated between putative membrane protein cboL, encoding methyltransferase, integration site Isolates could be clearly distinguished all known species their biovars means phenotypic molecular properties, therefore represent novel Brucella, for which name microti sp. nov. type strain (=BCCN 07-01T=CAPM 6434T) is proposed.