Halobacillus alkaliphilus sp. nov., a halophilic bacterium isolated from a salt lake in Fuente de Piedra, southern Spain

• DOI: 10.1099/ijs.0.65457-0
• Publication Date: 2008-04-08T23:03:27Z
• Authors: I. Romano; I. Finore; G. Nicolaus; F. J. Huertas; L. Lama; B. Nicolaus; A. Poli
• Keywords: Salt lake

A Gram-positive, spore-forming, halophilic bacterial strain, FP5T, was isolated from a salt lake in southern Spain and subjected to polyphasic taxonomic study. Strain FP5T strictly aerobic. Cells were coccoidal, occurring singly or clusters. The cell-wall peptidoglycan type of strain A4β based on l-Orn–d-Asp. characterized chemotaxonomically by having MK-7 as the major menaquinone anteiso-C15 : 0, anteiso-C17 iso-C15 0 iso-C16 main fatty acids. isolate grew optimally at 37 °C presence 10 % NaCl; no growth observed absence NaCl. DNA G+C content 43.5 mol%. Phylogenetic analyses 16S rRNA gene sequences showed that falls within evolutionary radiation species genus Halobacillus. Levels sequence similarity between strains nine recognized Halobacillus range 97.0–99.0 %. DNA–DNA relatedness indicated represents genomic is distinct species. could be differentiated several phenotypic characteristics. On basis phenotypic, phylogenetic data, considered represent novel Halobacillus, for which name alkaliphilus sp. nov. proposed. (=DSM 18525T =ATCC BAA-1361T).