Porphyromonas loveana sp. nov., isolated from the oral cavity of Australian marsupials

• DOI: 10.1099/ijsem.0.000898
• Publication Date: 2016-08-11T07:12:33Z
• Authors: Philip S. Bird; Darren J. Trott; Deirdre Mikkelsen; Gabriel J. Milino...; Kristine M. Hillman; Paul C. Burrell; Linda L. Blackall
• Keywords: MALDI-TOF; DNA, Bacterial; Evolution; 1105 Ecology; 03 medical and health sciences; Behavior and Systematics; Glutamate Dehydrogenase; RNA, Ribosomal, 16S; DNA, Ribosomal Spacer; Marsupial; Animals; Porphyromonas; 16S rRNA gene sequencing; Phylogeny; Base Composition; Mouth; 0303 health sciences; Pigmentation; 2404 Microbiology; Australia; Glutamate dehydrogenase gene sequencing; marsupial; Sequence Analysis, DNA; glutamate dehydrogenase gene sequencing; Bacterial Typing Techniques; 16S-23S rRNA internal transcribed spacer region sequencing; Marsupialia; MLST

An obligatory anaerobic, Gram-stain-negative coccobacillus with black-pigmented colonies was isolated from the oral cavity of selected Australian marsupial species. Phenotypic and molecular criteria showed that this bacterium was a distinct species within the genus Porphyromonas, and was closely related to Porphyromonas gingivalis and Porphyromonas gulae. This putative novel species and P. gulae could be differentiated from P. gingivalis by catalase activity. Further characterization by multi-locus enzyme electrophoresis of glutamate dehydrogenase and malate dehydrogenase enzyme mobility and matrix-assisted laser desorption ionization time-of-flight MS showed that this putative novel species could be differentiated phenotypically from P. gingivalis and P. gulae. Definitive identification by 16S rRNA gene sequencing showed that this bacterium belonged to a unique monophyletic lineage, phylogenetically distinct from P. gingivalis (94.9 % similarity) and P. gulae (95.5 %). This also was supported by 16S-23S rRNA intergenic spacer region and glutamate dehydrogenase gene sequencing. A new species epithet, Porphyromonas loveana sp. nov., is proposed for this bacterium, with DSM 28520T (=NCTC 13658T=UQD444T=MRK101T), isolated from a musky rat kangaroo, as the type strain.