Nesterenkonia aurantiaca sp. nov., an alkaliphilic actinobacterium isolated from Antarctica

• DOI: 10.1099/ijsem.0.000917
• Publication Date: 2016-01-26T17:12:27Z
• Authors: Ilaria Finore; Pierangelo Orlando; Paola Di Donato; Luigi Leone; Barbara Nicolaus; Annarita Poli
• Keywords: 0303 health sciences; 03 medical and health sciences; Nesterenkonia; Antarctica

A Gram-stain-positive, non-endospore-forming, haloalkaliphilic actinobacterium, strain CK5T, was isolated from a soil sample, collected at Cape King (Antarctica), and its taxonomic position investigated by using polyphasic approach. Cells were cocci with orange pigmentation, non-motile grew optimally 25 °C pH 9.0-9.5 in the presence of 2 % (w/v) NaCl. Cellular membrane contained MK-7 (72 %) MK-8 (28 %), anteiso-C15 : 0 (64.8 iso-C16 (13.3 n-C17 (9.9 n-C16 (4.0 n-C14 (3.7 as major cellular fatty acids. The DNA G+C content 64.8 mol%. Strain based on 16S rRNA gene sequence similarity, most closely related to Nesterenkonia jeotgali JG-241T (99.5 sandarakina YIM 70009T (99.4 lutea 70081T halotolerans 70084T (99.3 xinjiangensis 70097T (97.2 flava CAAS 251T (97.1 Nesterekonia aethiopica CCUG 48939T %). CK5T revealed 31 DNA-DNA relatedness respect N. DSM 15664T, 29 19081T, 10 15666T 1 halotolerans, 15474T, 15475T, 17733T 19422T. On basis sequences, hybridization chemotaxonomic characteristics, represents novel species genus Nesterenkonia, for which name aurantiaca sp. nov. is proposed. type ( = 27373T JCM 19723T).