Three novel ascomycetous yeast species of the Kazachstania clade, Kazachstania saulgeensis sp. nov., Kazachstania serrabonitensis sp. nov. and Kazachstania australis sp. nov. Reassignment of Candida humilis to Kazachstania humilis f.a. comb. nov. and Candida pseudohumilis to Kazachstania pseudohumilis f.a. comb. nov.

• DOI: 10.1099/ijsem.0.001495
• Publication Date: 2016-10-13T07:12:59Z
• Authors: Noémie Jacques; Véronique Sarilar; Charlotte Urien; Mariana R. Lopes; Camila G. Morais; Ana Paula T. Ueta...; Colin R. Tinsley; Carlos A. Rosa; Delphine Sicard; Serge Casaregola
• Keywords: Exigua

Five ascosporogenous yeast strains related to the genus Kazachstania were isolated. Two (CLIB 1764T and CLIB 1780) isolated from French sourdoughs; three others (UFMG-CM-Y273T, UFMG-CM-Y451 UFMG-CM-Y452) rotting wood in Brazil. The sequences of Brazilian differed by one substitutions, respectively, D1/D2 large subunit (LSU) rRNA gene internal transcribed spacer (ITS). LSU sequence these 0.5 0.7 % exigua, but their ITS diverged 8.1 8.3 %, that closest described species barnettii. Analysis protein coding RPB1, RPB2 EF-1α distinguished strains, with respectively 3.3, 6 11.7 substitutions. novel are accommodate newly strains: saulgeensis sp. nov. (type strain 1764T=CBS 14374T) serrabonitensis UFMG-CM-Y273T=CLIB 1783T=CBS 14236T). Further analysis culture collections revealed a previously assigned K. exigua species, having 3.8 difference (22 substitutions 2 indels) its respect exigua. Hence, we describe new taxon, australis 162T=CBS 2141T), this strain. Finally, Candida humilis pseudohumilis reassigned as combinations. On basis analysis, also propose milleri comb. conspecific.