Azoarcus halotolerans sp. nov., a novel member of Rhodocyclaceae isolated from activated sludge collected in Hong Kong

• DOI: 10.1099/ijsem.0.004476
• Publication Date: 2020-09-18T12:26:34Z
• Authors: Shuyang Li; Lin Zhao; Jingyi Han; Shuangyuan Liu; Jingcheng Dai; Guiping Fu; Dongru Qiu
• Keywords: genomic DNA; Strain (injury); Phosphatidylglycerol

A floc-forming bacterial strain, designated HKLI-1 T , was isolated from the activated sludge of a municipal sewage treatment plant in Hong Kong SAR, PR China. Cells this strain were Gram-stain-negative, strictly aerobic, catalase- and oxidase-positive, rod-shaped motile by means single polar flagellum. Growth occurred at 18–37 °C (optimum, 28 °C), pH 5.5–9.0 7.5) with 0–8.0 % (w/v) NaCl 1–1.5 %) concentration. The major fatty acids C 16 : 0 summed feature 3 (C 1 ω7 c and/or ω6 ). lipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol three unidentified lipids. DNA G+C content 63.5 mol% whole genomic sequence analysis. Based on results 16S rRNA gene sequences analysis, should be assigned to genus Azoarcus is closely related olearius DQS-4 (94.93 pairwise similarity), toluclasticus MF63 (94.91 communis SWub3 (94.01 %), but separate them large distances different phylogenetic trees. genome orthologous average nucleotide identity silico DNA–DNA hybridization values against four closest relatives 73.03–74.83 17.2–23.0 %, respectively. phylogenetic, genotypic, phenotypic chemotaxonomic data demonstrated that could distinguished its phylogenetically species, represented novel species within for which name halotolerans sp. nov. proposed. type (= 72659 =CCTCC AB 2019312