Lacticaseibacillus parakribbianus sp. nov., isolated from a pig farm faeces dump

• DOI: 10.1099/ijsem.0.005758
• Publication Date: 2023-03-21T16:20:46Z
• Authors: Lu Bai; Jayoung Paek; Yeseul Shin; Hongik Kim; Si Hyun Kim; Jeong Hwan Shin; Joong-Ki Kook; Young Hyo Chang
• Keywords: DNA, Bacterial; Base Composition; Farms; Swine; Fatty Acids; Sequence Analysis, DNA; Peptidoglycan; Bacterial Typing Techniques; Feces; Lacticaseibacillus; RNA, Ribosomal, 16S; Animals; Phylogeny; Phospholipids

A lactic acid bacterium isolated from pig faeces was characterized using a polyphasic approach. The strain was Gram-stain-positive, rod-shaped, and facultative anaerobic. Phylogenetic analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the genus

Lacticaseibacillus

. The multi-locus sequence tree revealed that the strain formed a sub-cluster adjacent to Lacticaseibacillus kribbianus. The main fatty acids were C16 : 0 and C18 : 1ω9c. The average nucleotide identity value, average amino acid identity, and genome-to-genome distance for YH-lacS6T and its most closely related strain, L. kribbianus, were 85.4, 85.2 and 29.2 %, respectively. The G+C content of the genomic DNA was 61.6 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, aminophospholipids and phospholipids. The cell-wall peptidoglycan did not contain meso-diaminopimelic acid. Thus, YH-lacS6T (=KCTC 21186T=JCM 34954T) represents a novel species. The name Lacticaseibacillus parakribbianus sp. nov. is proposed.