Parachitinimonas caeni gen. nov., sp. nov., a novel member of family Burkholderiaceae isolated from activated sludge collected in Shenzhen, PR China

• DOI: 10.1099/ijsem.0.006249
• Publication Date: 2024-01-29T13:16:58Z
• Authors: Xin Wang; Han Chen; Jing Wang; Siliang Yuan; Dian Jiao; Shanhui Wang; Yaqi Liu; Yan Li; Jingcheng Dai; Shuyang Li; Dongru Qiu
• Keywords: Strain (injury); genomic DNA

A Gram-stain-negative, strictly aerobic and filamentous bacterial strain, designated as DQS-5 T , was isolated from the activated sludge of a municipal sewage treatment plant in Shenzhen, PR China. Optimal growth observed at 28 °C pH 7.5. Catalase oxidase activities were detected. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain most closely related to genera Chitinimonas Chitinivorax (91.0–93.4 % 92.5 sequence similarity, respectively) close member family Burkholderiaceae . The complete genome contains 5 653 844 bp 57.3 mol% G+C. average nucleotide identity, digital DNA–DNA hybridization amino acid identity values between those its relatives 75.9–77.2, 19.0–20.3 57.2–61.8 %, respectively. Chemotaxonomic indicated sole respiratory quinone ubiquinone-8, predominant cellular fatty acids C 16 : 0 summed feature 3 (C 1 ω7 c and/or ω6 ), major polar lipids consisted phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid aminolipid. phylogenetic, genotypic, phenotypic chemotaxonomic data demonstrate represents novel species genus within for which name Parachitinimonas caeni gen. nov., sp. is proposed. Strain (=KCTC 92788 =CCTCC AB 2022320 ) type only P.