Oyster viperin retains direct antiviral activity and its transcription occurs via a signalling pathway involving a heat-stable haemolymph protein

• DOI: 10.1099/jgv.0.000300
• Publication Date: 2015-09-25T20:12:20Z
• Authors: Timothy J. Green; Peter Speck; Lu Geng; David Raftos; Michael R. Beard; Karla J. Helbig
• Keywords: Transcription; TLR3; Heterologous; Antiviral protein; Hemolymph

Little is known about the response of non-model invertebrates, such as oysters, to virus infection. The vertebrate innate immune system detects virus-derived nucleic acids trigger type I IFN pathway, leading transcription hundreds IFN-stimulated genes (ISGs) that exert antiviral functions. Invertebrates were thought lack pathway based on absence or ISGs encoded in model invertebrate genomes. However, oyster genome encodes many ISGs, including well-described protein viperin. In this study, we characterized viperin and showed it localizes caveolin-1 inhibits dengue replication a heterologous model. second set experiments, have provided evidence haemolymph from poly(I : C)-injected oysters contains heat-stable, protease-susceptible factor induces haemocyte mRNA conjunction with upregulation regulatory factor. Collectively, these results support concept systems are homologous pathway.