Identification and characterization of HolGH15: the holin of Staphylococcus aureus bacteriophage GH15

• DOI: 10.1099/jgv.0.000428
• Publication Date: 2016-02-12T16:40:00Z
• Authors: Jun Song; Feifei Xia; Haiyan Jiang; Xinwei Li; Liyuan Hu; Pengjuan Gong; Liancheng Lei; Xin Feng; Changjiang Sun; Jingmin Gu; Wenyu Han
• Keywords: Gene Expression Regulation, Viral; 0301 basic medicine; Cytoplasm; Staphylococcus aureus; Membrane Proteins; Genome, Viral; Underage Drinking; Listeria monocytogenes; Anti-Bacterial Agents; 3. Good health; Viral Proteins; 03 medical and health sciences; Mutation; Amino Acid Sequence; Cloning, Molecular; Staphylococcus Phages

Holins are phage-encoded hydrophobic membrane proteins that spontaneously and non-specifically accumulate form lesions in the cytoplasmic membrane. The ORF72 gene (also designated HolGH15) derived from genome of Staphylococcus aureus phage GH15 was predicted to encode a protein. An analysis indicated protein encoded by HolGH15 potentially consisted two transmembrane helices. This exhibited structural characteristics class II holins belonged phage_holin_1 superfamily. Expression Escherichia coli BL21 cells resulted growth retardation host cells, which triggered prematurely addition 2,4-dinitrophenol. expression caused morphological alterations engineered E. including loss cell wall integrity release intracellular components, were visualized transmission electron microscopy. exerted efficient antibacterial activity at 37 °C pH 5.2. Mutation domains indispensable for full-length showed broad range: it not only inhibited aureus, but also demonstrated against other species, Listeria monocytogenes, Bacillus subtilis, Pseudomonas aeruginosa, Klebsiella pneumoniae coli. At minimal inhibitory concentration, evoked cellular contents shrinkage death monocytogenes cells. To best our knowledge, this study is first report holin exerts heterogeneous pathogens.