Eugenol in combination with lactic acid bacteria attenuates Listeria monocytogenes virulence in vitro and in invertebrate model Galleria mellonella

• DOI: 10.1099/jmm.0.000251
• Publication Date: 2016-03-22T22:12:22Z
• Authors: Abhinav Upadhyay; Indu Upadhyaya; Shankumar Mooyottu; Kumar Venkitanara...
• Keywords: Listeriolysin O; Galleria mellonella

Listeria monocytogenes is a human enteric pathogen that causes severe foodborne illness in high-risk populations. Crossing the intestinal barrier first critical step for infection. Therefore, reducing L. colonization and invasion of epithelium production virulence factors could potentially control listeriosis humans. This study investigated efficacy sub-inhibitory concentration (SIC) plant-derived antimicrobial eugenol, either alone, or combination with five lactic acid bacteria (LAB), namely Bifidobacterium bifidum (NRRL-B41410), Lactobacillus reuteri (B-14172), fermentum (B-1840), plantarum (B-4496) Lactococcus lactis subspecies (B-633) adhesion to epithelial cells (Caco-2). Additionally, effect aforementioned treatments on listeriolysin production, E-cadherin binding expression genes was investigated. Moreover, vivo eugenol-LAB invertebrate model Galleria mellonella studied. Eugenol LAB, alone combination, significantly reduced (P < 0.05). decreased haemolysin gene In addition, enhanced survival rates G. infected lethal doses The results highlight antilisterial eugenol justify further investigations mammalian model.