Evaluation of Xpert MRSA Gen 3 and BD MAX MRSA XT for meticillin-resistant Staphylococcus aureus screening in a routine diagnostic setting in a low-prevalence area

• DOI: 10.1099/jmm.0.000411
• Publication Date: 2016-12-20T06:12:39Z
• Authors: Xiaohui Chen Nielsen; Tina Vasehus Madsen; Jørgen Engberg
• Keywords: Isolation; Meticillin

Screening and pre-emptive isolation of at-risk patients are important aspects the Danish approach to prevention meticillin-resistant Staphylococcusaureus (MRSA) infection, but screening with conventional culture can take up 3 days for results become available attendant costs disadvantages prolonged isolation. We sought evaluate accuracy, time availability potential economic benefits two next-generation MRSA assays, Xpert Gen (GX MRSA) BD MAX XT, in a setting consolidated laboratory serving number hospitals low prevalence using enrichment as reference method. Four hundred forty-seven samples together 49 previously positive were evaluated. demonstrated sensitivity, specificity, predictive value negative 88.2, 97.9, 62.5 99.5 %, respectively, they 97.4, 57.7 respectively. Hands-on was 8.8 21.6 min, XT PCR assays when five handled simultaneously. The mean turnaround 2.9 (1-6) hours assay, 6.5 (2-46) 49.6 (42-122) enriched culture. Despite being higher rapid taken into account, offer significant cost savings.