Synergistic potential of Juniperus communis and Helichrysum italicum essential oils against nontuberculous mycobacteria

• DOI: 10.1099/jmm.0.000962
• Publication Date: 2019-03-27T12:37:03Z
• Authors: Dolores Peruč; Brigita Tićac; Maja Abram; Dalibor Broznić; Sanja Štifter; Mladenka Malenica...; Ivana Gobin
• Keywords: Antimycobacterial; Nontuberculous Mycobacteria; Minimum bactericidal concentration

The present study evaluated the possible synergistic antimycobacterial interactions of Juniperus communis and Helichrysum italicum essential oils (EO).Antimycobacterial potential was tested against Mycobacterium avium intracellulare using broth water dilution method checkerboard synergy method. Antiadhesion antibiofilm effect EOs on biotic (HeLa cells) abiotic surface (polystyrene). To evaluate mechanisms action, cellular leakage proteins DNA structural changes were visualized with a transmission electron microscope.MIC, minimum bactericidal concentration (MBC) minimal effective (MEC) 1.6 mg ml-1 for J. EO 3.2 H. both mycobacteria. All combinations in produced fractional inhibitory index values ranging from 0.501 to 1.5, corresponding synergistic, additive or indifferent effects. showed greater tendency create biofilm but these at subinhibitory concentrations (sMIC) effectively blocked adhesion establishment biofilm. exposure mycobacteria MICs sMICs lead significant morphological changes: acquired swollen form, ghost-like cell, disorganized cytoplasm detached cell wall. OD value supernatant exposed have confirmed that there is material.The material noticeably higher sMIC, which probably due wall damage. sMIC an effect, reducing MICs, limiting preventing formation biofilms.