Comparison of various antimicrobial agents as catheter lock solutions: preference for ethanol in eradication of coagulase-negative staphylococcal biofilms

• DOI: 10.1099/jmm.0.006387-0
• Publication Date: 2009-03-09T19:18:01Z
• Authors: Yue Qu; Taghrid S. Istivan; Andrew J. Daley; Duncan A. Rouch; Margaret A. Deighton
• Keywords: Coagulase

Coagulase-negative staphylococci (CoNS) are the main causative agents of bacteraemia in infants managed neonatal intensive care units (NICUs). Intraluminal colonization long-term central venous catheters by these bacteria and subsequent biofilm formation prerequisites bloodstream infections acquired NICUs. The catheter lock technique has been used to treat colonization; however, optimum choice antimicrobial their corresponding concentrations exposure times have not determined. effectiveness solutions (CLSs) was assessed determining minimal eradication concentration against CoNS biofilms. Five conventional antibiotics (oxacillin, gentamicin, vancomycin, ciprofloxacin rifampicin) alone or combination, as well ethanol, were evaluated. Ethanol found be superior all when a CLS. A time-kill study confocal laser scanning microscopy revealed that 40 % ethanol for 1 h sufficient kill cells. To our knowledge, this is first vitro provide solid evidence support rationale using at low short time CLS, instead high long period catheter-related infections.