The changes of PCR ribotype and antimicrobial resistance of Clostridium difficile in a tertiary care hospital over 10 years

• DOI: 10.1099/jmm.0.072082-0
• Publication Date: 2014-04-03T00:21:44Z
• Authors: Jong-Han Lee; Yangsoon Lee; Kyungwon Lee; Thomas V. Riley; Heejung Kim
• Keywords: Ribotyping

The aims of this study were to investigate any change in PCR ribotypes and determine the antimicrobial resistance common over a 10-year period tertiary care hospital. We conducted ribotyping, susceptibility testing DNA gyrase sequencing identify changes 1407 Clostridium difficile non-duplicated isolates obtained between 2000 2009. A total 74 different found. most prevalent ribotype was 001 (26.1 %). prevalence 017 17 % that 014/020 9.6 %. Ribotyping showed decreased 017, 018 increased 10 years. Antimicrobial rates were: clindamycin, 81 %; cefotetan, 19 moxifloxacin, 42 imipenem, 8 ciprofloxacin, 100 erythromycin, 80 Ribotype greater than other ribotypes. All strains showing moxifloxacin had substitution gyrA coding amino acid (Thr82 Ile). This will help understanding trends C. Korea.