Different scenarios for Candida parapsilosis fungaemia reveal high numbers of mixed C. parapsilosis and Candida orthopsilosis infections

• DOI: 10.1099/jmm.0.080655-0
• Publication Date: 2014-10-29T07:44:47Z
• Authors: Leonardo Silva Ba...; Catarina Vaz; Célia Pais; Maria Helena Gald...; Mauro de Medeiros...; Rosely Maria Zanc...; Paula Sampaio
• Keywords: Candida parapsilosis; Fungemia

Nosocomial fungal bloodstream infections (BSI) are increasing significantly in hospitalized patients and Candida parapsilosis has emerged as an important pathogen responsible for numerous outbreaks. The objective of this study was to evaluate C. sensu lato infection scenarios, regarding species distribution strain relatedness. One hundred isolates derived from blood cultures catheter tips were analysed by multiplex microsatellite typing sequencing D1/D2 regions the ribosomal DNA. Our results indicate that 9.5 % presented due orthopsilosis, 57.1 parapsilosis, 28.3 orthopsilosis 4.8 metapsilosis. Eighty per cent BSIs a single also identified catheter, but 10 cases parasilosis BSI orthopsilosis. There is significant probability collected same patient at more than 3 months interval different strains (P = 0.0179). Moreover, several persistently hospital, infecting six patients. incidence polyfungal with reported herein first time, emphasizing fact not always BSI, thus impacting treatment strategy. observation can remain hospital environment years highlights possible existence reservoirs reinforces need accurate genotyping tools, such markers used elucidating epidemiological associations detecting