Bordetella holmesii DNA is not detected in nasopharyngeal swabs from Finnish and Dutch patients with suspected pertussis

• DOI: 10.1099/jmm.0.46331-0
• Publication Date: 2006-07-18T23:08:08Z
• Authors: Mia Antila; Qiushui He; Caroline de Jong; Ingrid Aarts; Harold Verbakel; Sylvia Bruisten; Suzanne Keller; Marjo Haanperä; Johanna Mäkinen; Erkki Eerola; Matti K. Viljanen; Jussi Mertsola; Anneke van der Zee
• Keywords: Whooping cough; Bordetella

Bordetella holmesii is a Gram-negative bacterium first identified in 1995. It can cause pertussis-like symptoms humans. B. contains insertion sequences IS481 and IS1001, two frequently used targets the PCR diagnosis of pertussis parapertussis infections. To investigate prevalence Finnish Dutch patients with whether has caused any false-positive results diagnostic PCRs, holmesii-specific real-time PCRs were developed. The methods conventional (LightCycler, Roche) targeting recA gene. IS1001 or formats homologue IS1001. Of 11,319 nasopharyngeal swabs, 2804 collected from 2000 to 2003, 8515 1992 2003. DNA was not found samples analysed. suggest that among causative agents thus does practice confound PCRs.