Formation and properties of in vitro biofilms of ica-negative Staphylococcus epidermidis clinical isolates

• DOI: 10.1099/jmm.0.46799-0
• Publication Date: 2006-12-15T19:51:25Z
• Authors: Zhiqiang Qin; Xiaomei Yang; Lei Yang; Juan Jiang; Yuanzhu Ou; Soeren Molin; Di Qu
• Keywords: Lysostaphin

Coagulase-negative Staphylococcus epidermidis has become the leading cause of foreign-body infections due to its biofilm formation on all kinds medical-device surfaces. The development S. includes two steps: initial attachment phase and accumulative phase. In phase, polysaccharide intercellular adhesin (PIA), encoded by icaADBC locus, is major component mediating adhesion. However, recent studies have revealed emergence biofilm-positive/ ica -negative staphylococcal clinical isolates. this report, strains, SE1 SE4, exhibited their heterogeneity in architecture under static flow conditions, compared with -positive RP62A strain. Strains type absence PIA from biofilms also displayed intermediate resistance vancomycin. More importantly, cells both SE4 strains were more tolerant than those exposure lysostaphin Based results, it suggested that strain represents a newly emergent subpopulation arising selection antibiotics nosocomial milieu, which displays survival advantage host environment. Recent epidemiological data support suggestion, showing tendency towards an increasing proportion staphylococci-associated infections.