Detection of mixed clarithromycin-resistant and -susceptible Helicobacter pylori using nested PCR and direct sequencing of DNA extracted from faeces

• DOI: 10.1099/jmm.0.47302-0
• Publication Date: 2007-08-29T17:28:00Z
• Authors: Norihisa Noguchi; Emiko Rimbara; Ayami Kato; Akifumi Tanaka; Kengo Tokunaga; Takashi Kawai; Shin'ichi Takahashi; Masanori Sasatsu
• Keywords: 23S ribosomal RNA; Chronic gastritis

The major cause of chemotherapy failure in patients with chronic gastritis and peptic ulcers caused by Helicobacter pylori is clarithromycin (CAM) resistance due to a mutation the 23S rRNA gene. This study describes non-invasive accurate method for detection mixed CAM-resistant -susceptible H. sequencing Faeces were crushed beads gene was amplified using nested PCR on extracted DNA. Mutation analysis this showed that 20.4 % carried CAM-susceptible (wild type) -resistant (A2142G or A2143G mutant) pylori. Furthermore, it found 66.6 who had been treated unsuccessfully one these mutations (including type), whilst standard culture detected isolates only 22.2 unsuccessful treatment. These data suggest that, successful therapy, diagnosis described here would more accurately detect pylori, including infections.