The characterization of mobile colistin resistance (mcr) genes among 33 000 Salmonella enterica genomes from routine public health surveillance in England

Salmonella enterica Resistome Colistin MCR-1
DOI: 10.1099/mgen.0.000331 Publication Date: 2020-01-31T16:13:25Z
ABSTRACT
To establish the prevalence of mobile colistin resistance (mcr) genes amongst Salmonella enterica isolates obtained through public health surveillance in England (April 2014 to September 2017), 33 205 S. genome sequences from human, food, animal and environmental were screened for presence mcr variants 1 8. The mcr-positive genomes assembled, annotated characterized according plasmid type. Nanopore sequencing was performed on six selected with putative novel plasmids, phylogenetic analysis used provide an evolutionary context most commonly isolated clones. Fifty-two identified, which 32 positive mcr-1, 19 mcr-3 mcr-5. combination Illumina identified three plasmids one mcr-5 plasmid, as well chromosomally integrated mcr-1 mcr-3. Monophasic serovar Typhimurium accounted 27/52 (52 %) isolates, majority clustering clades associated travel Southeast Asia. Isolates these a specific range additional extended-spectrum beta-lactamase genotype. Routine whole-genome provides effective screen emerging antimicrobial determinants, including mcr. Complementary long-read technologies elucidated genomic offering insights into dissemination linkage other genes.
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