Whole-genome sequencing of Chlamydia psittaci from Australasian avian hosts: A genomics approach to a pathogen that still ruffles feathers

• DOI: 10.1099/mgen.0.001072
• Publication Date: 2023-07-24T16:17:36Z
• Authors: Vasilli Kasimov; Rhys T. White; Jonathan Foxwell; Cheryl Jenkins; Kristene Gedye; Yvonne Pannekoek; Martina Jelocnik
• Keywords: Chlamydia psittaci

Chlamydia psittaci is a globally distributed veterinary pathogen with zoonotic potential. Although C. infections have been reported in various hosts, isolation and culture of challenging, hampering efforts to produce contemporary global genomes. This particularly evident the lack avian genomes from Australia New Zealand. In this study, we used culture-independent probe-based whole-genome sequencing expand genome catalogue. Here, provide new two pigeons, six psittacines, novel hosts such as Australian bustard (Ardeotis australis) sooty shearwater (Ardenna grisea) We also evaluated genetic diversity using multilocus sequence typing (MLST) major outer membrane protein (ompA) genotyping on additional psittaci-positive samples captive field isolates Australasia. showed that first sequenced Zealand parrots pigeons belong clonal type (ST)24 diverse 'pigeon-type' ST27 clade, respectively. parrot-derived strains clustered ST24 group, whereas ST332 strain genetically clade fulmar, parrot, livestock. MLST ompA revealed ST24/ompA genotype A wild shearwater, whilst 'pigeon-types' (ST27/35 genotypes B/E) were found other atypical parrots, little blue penguin/Kororā (Eudyptula minor) zebra finch (Taeniopygia guttata castanotis) study provides insights into phylogenomic further demonstrates multi-host generalist capacity pathogen.