The function of the three phosphoribosyl pyrophosphate synthetase (Prs) genes in hyphal growth and conidiation in Aspergillus nidulans

• DOI: 10.1099/mic.0.000427
• Publication Date: 2017-01-13T23:12:49Z
• Authors: Ping Jiang; Wen-fan Wei; Guo-wei Zhong; Xiao-gang Zhou; Wei-ran Qiao; Reinhard Fisher; Ling Lu
• Keywords: Conidiation; Aspergillus nidulans

Phosphoribosyl pyrophosphate synthetase, which is encoded by the Prs gene, catalyses reaction of ribose-5-phosphate and adenine ribonucleotide triphosphate (ATP) has central importance in cellular metabolism. However, knowledge about how family members function contribute to total 5-phosphoribosyl-α-1-pyrophosphate (PRPP) synthetase activity limited. In this study, we identified that filamentous fungus Aspergillus nidulans genome contains three PRPP synthase-homologous genes (AnprsA, AnprsB AnprsC), among AnprsC but not AnprsA are auxotrophic genes. Transcriptional expression profiles revealed mRNA levels AnprsA, Anprs C dynamic during germination, hyphal growth sporulation they all showed abundant vigorous time point. Inhibiting or conditional strains produced more effects on than did inhibiting thus indicating different AnPrs proteins unequal their contributions enzyme activity. addition, constitutive overexpression could significantly rescue defective phenotype AnprsB-absent strain, suggesting a specific consequence gene instead comes from contribution