A distinct physiological role of MutY in mutation prevention in mycobacteria

• DOI: 10.1099/mic.0.033621-0
• Publication Date: 2009-11-03T22:52:21Z
• Authors: Krishna Kurthkoti; Thiruneelakantan ...; Pradeep Kumar; Vidyasagar S. Mal...; Pau Biak Sang; Ruchi Jain; Ramanathapuram Ma...; Umesh Varshney
• Keywords: Microbiology & Cell Biology; DNA, Bacterial; 0301 basic medicine; Guanine; DNA Repair; Mycobacterium smegmatis; 500; Hydrogen Peroxide; Biochemistry; Cell Line; DNA Glycosylases; Substrate Specificity; 3. Good health; Mice; 03 medical and health sciences; Bacterial Proteins; Mutation; Animals; Rifampin; DNA Damage

Oxidative damage to DNA results in the occurrence of 7,8-dihydro-8-oxoguanine (8-oxoG) genome. In eubacteria, repair such is initiated by two major base-excision enzymes, MutM and MutY. We generated a MutY-deficient strain Mycobacterium smegmatis investigate role this enzyme repair. The MutY deficiency M. did not result either noteworthy susceptibility oxidative stress or an increase mutation rate. However, rifampicin-resistant isolates showed distinct mutations rifampicin-resistance-determining region rpoB . Besides expected C A (or G T) mutations, T G) was also observed. Biochemical characterization mycobacterial ( tuberculosis ) revealed excision opposite 8-oxoG DNA. Additionally, detected. formed complexes with containing : A, but pairs. Primer extension reactions cell-free extracts suggested error-prone incorporation nucleotides into Based on these observations, we discuss physiological specific prevention mycobacteria.