Infection of the Circulifer haematoceps cell line Ciha-1 by Spiroplasma citri: the non-insect-transmissible strain 44 is impaired in invasion

• DOI: 10.1099/mic.0.035063-0
• Publication Date: 2009-12-18T03:46:03Z
• Authors: Sybille Duret; Brigitte Batailler; Jean-Luc Danet; Laure Béven; Joël Renaudin; Nathalie Arricau-...
• Keywords: Spiroplasma; Strain (injury)

Successful transmission of Spiroplasma citri by its leafhopper vector requires a specific interaction between the spiroplasma surface and insect cells. With aim studying these interactions at cellular molecular levels, cell line, named Ciha-1, was established using embryonic tissues from eggs S. natural Circulifer haematoceps. This is first report, to our knowledge, line for this species successful infection insect-transmissible strain GII3. Adherence spiroplasmas cultured Ciha-1 cells studied c.f.u. counts electron microscopy. Entry into analysed quantitatively gentamicin protection assays qualitatively double immunofluorescence Spiroplasmas were detected within cytoplasm as early 1 h after inoculation survived least 2 days inside Comparing GII3 non-insect-transmissible 44 strains revealed that adherence entry significantly less efficient than those