The ATPases CopA and CopB both contribute to copper resistance of the thermoacidophilic archaeon Sulfolobus solfataricus

• DOI: 10.1099/mic.0.055905-0
• Publication Date: 2012-02-24T03:24:48Z
• Authors: Christian Völlmecke; Steffen L. Drees; Julia Reimann; Sonja-Verena Albers; Mathias Lübben
• Keywords: Sulfolobus solfataricus; Sulfolobus

Certain heavy metal ions such as copper and zinc serve essential cofactors of many enzymes, but are toxic at high concentrations. Thus, intracellular levels have to be subtly balanced. P-type ATPases the P(IB)-subclass play a major role in homeostasis. The thermoacidophile Sulfolobus solfataricus possesses two P(IB)-ATPases named CopA CopB. Both enzymes present cells grown copper-depleted medium accumulated upon an increase external concentration. We studied physiological roles both by disrupting genes copA copB. Neither them affected sensitivity S. reactive oxygen species, nor were they strict prerequisite biosynthesis protein cytochrome oxidase. Deletion mutant analysis demonstrated that is effective pump low CopB appeared low-affinity export ATPase, which was only relevant if media concentration exceedingly high. thus act resistance factors overlapping Moreover, growth tests on solid indicated involved silver.