Occurrence of mutations impairing sigma factor B (SigB) function upon inactivation of Listeria monocytogenes genes encoding surface proteins

• DOI: 10.1099/mic.0.067744-0
• Publication Date: 2013-05-09T04:01:55Z
• Authors: Juan J. Quereda; M. Graciela Pucci...; Laura Botello-Morte; Enrique Calvo; Filipe Carvalho; Christiane Bouchier; Ana Vieira; Javier F. Mariscotti; Trinad Chakraborty; Pascale Cossart; Torsten Hain; Didier Cabanes; Francisco García-...
• Keywords: Sigma factor; Proteome; Cell envelope; Loss function

Bacteria of the genus Listeria contain largest family LPXTG surface proteins covalently anchored to peptidoglycan. The extent which these may function or be regulated cooperatively is at present unknown. Because their unique cellular location, we reasoned that distinct could act as elements contributing cell wall homeostasis influencing stability other bound To test this hypothesis, used proteomics analyse mutants intracellular pathogen monocytogenes lacking implicated in pathogen-host interactions, such InlA, InlF, InlG, InlH, InlJ, LapB and Vip. Changes proteome were found inlG vip mutants, exhibited reduced levels Lmo0610, Lmo0880 Lmo2085, all by stress-related sigma factor SigB. ultimate basis alteration was uncovered genome sequencing, revealed carried loss-of-function mutations rsbS, rsbU rsbV genes encoding regulatory control SigB activity. Attempts recapitulate negative selection a large series new constructed for purpose were, however, unsuccessful. These results indicate inadvertent secondary affecting functionality can randomly arise L. when using common genetic procedures during subculturing. Testing activity therefore valuable manipulating genetically prior any subsequent phenotypic analysis. This even more justified generating deletions envelope components.