Francisella tularensis subsp. holarctica DsbA homologue: a thioredoxin-like protein with chaperone function

• DOI: 10.1099/mic.0.070516-0
• Publication Date: 2013-09-07T02:42:30Z
• Authors: Monika Schmidt; Jana Klimentova; Pavel Rehulka; Adela Straskova; Petra Spidlova; Barbora Szotakova; Jiri Stulik; Ivona Pavkova
• Keywords: DsbA; FKBP; Chaperone (clinical)

Francisella tularensis is a highly infectious facultative intracellular bacterium and aetiological agent of tularaemia. The conserved hypothetical lipoprotein with homology to thiol/disulphide oxidoreductase proteins (FtDsbA) an essential virulence factor in F. tularensis. Its protein sequence has two different domains: the DsbA_Com1_like domain (DSBA), catalytically active site CXXC cis-proline residue; amino-terminal FKBP-type peptidyl-prolyl isomerases (FKBP_N). To establish role both domains tularaemia infection models, site-directed deletion mutagenesis affecting (AXXA), (P286T) FKBP_N (ΔFKBP_N) were performed. generated mutations led high attenuation ability induce full or partial host protective immunity. Recombinant analysis revealed that as well residue are necessary for correct activity. By contrast, only DSBA (and not domain) seems be responsible vitro chaperone activity FtDsbA protein.