An aflagellate mutant Yersinia enterocolitica biotype 1A strain displays altered invasion of epithelial cells, persistence in macrophages, and cytokine secretion profiles in vitro

• DOI: 10.1099/mic.0.2006/000919-0
• Publication Date: 2007-04-26T22:57:57Z
• Authors: Alan McNally; Roberto M. La Rag...; Angus. Best; Georgina Manning; Diane G. Newell
• Keywords: Yersinia enterocolitica; Strain (injury)

Despite being classically defined as non-pathogenic, there is growing evidence that biotype 1A Yersinia enterocolitica isolates may be aetiological agents of disease in humans. In previous studies, a potential link between motility and the ability strains to invade cultured epithelial cells was observed. an attempt further investigate this finding, flagella mutant constructed human faecal Y. isolate. The mutation abolished strain cells, although adherence not affected. aflagellate also attenuated its survive within macrophages, cleared after 3 h, whilst wild-type persisted for 24 h infection. Examination cytokine secretion by infected macrophages suggested act anti-inflammatory agents, decreasing production tumour necrosis factor (TNF)-α increasing interleukin (IL)-10. Preliminary studies using porcine vitro organ culture (IVOC) tissue colonize intestinal tissue.