High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169

• DOI: 10.1099/vir.0.013250-0
• Publication Date: 2009-09-14T18:36:17Z
• Authors: Amanda J. Bradley; Nell S. Lurain; Peter Ghazal; Urmi Trivedi; Charles Cunningham; Katarina Baluchova; Derek Gatherer; Gavin W. G. Wilki...; Derrick J. Dargan; Andrew J. Davison
• Keywords: Cytomegalovirus; Sequence (biology)

The genomes of commonly used variants human cytomegalovirus (HCMV) strains Towne and AD169 each contain a substantial mutation in which region (U(L)/b') at the right end long unique has been replaced by an inverted duplication from left genome. Using high-throughput technology, we have sequenced HCMV strain (ATCC VR-977) confirmed presence two variants, one exhibiting replacement U(L)/b' other intact this region. Both are mutated genes RL13, UL1, UL40, UL130, US1 US9. We also novel variant (varUC) that is except for small deletion affects UL144, UL142, UL141 UL140. Like varUC RL5A, UL36 UL131A. A subpopulation contains additional affecting IRS1, US2.