Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs

• DOI: 10.1099/vir.0.014480-0
• Publication Date: 2009-08-12T19:43:27Z
• Authors: E. Lange; D. Kalthoff; U. Blohm; J. P. Teifke; A. Breithaupt; C. Maresch; E. Starick; S. Fereidouni; B. Hoffmann; T. C. Mettenleiter; M. Beer; T. W. Vahlenkamp
• Keywords: Seroconversion; Nucleoprotein; Viral Shedding

Influenza virus A/H1N1, which is currently causing a pandemic, contains gene segments with ancestors in the North American and Eurasian swine lineages. To get insights into replication dynamics, clinical symptoms transmission pigs, we infected animals intranasally influenza A/Regensburg/D6/09/H1N1. Virus excretion inoculated pigs was detected nasal swabs from 1 day post-infection (p.i.) onwards developed generally mild symptoms, including fever, sneezing, discharge diarrhoea. Contact became infected, shed similar to those animals. Plasma samples of all remained negative for RNA. Nucleoprotein- haemagglutinin H1-specific antibodies could be by ELISA 7 days p.i. CD4+ T cells activated immediately after infection both CD8+ T-cell populations expanded 3 p.i., coinciding signs. chickens uninfected, as judged absence excretion, signs seroconversion.