Comprehensive full-length sequence analyses of human parechoviruses: diversity and recombination

• DOI: 10.1099/vir.0.014670-0
• Publication Date: 2009-11-03T22:55:36Z
• Authors: K. S. M. Benschop; M. de Vries; R. P. Minnaar; G. Stanway; L. van der Hoek; K. C. Wolthers; P. Simmonds
• Keywords: Recombination, Genetic; 570; 0303 health sciences; Picornaviridae Infections; Genotype; QH301 Biology; Molecular Sequence Data; 610; Genetic Variation; Parechovirus; Genome, Viral; 3. Good health; 03 medical and health sciences; Cluster Analysis; Humans; RNA, Viral; Sequence Analysis; Phylogeny; Netherlands

Human parechoviruses (HPeVs) are highly prevalent pathogens among very young children. Although originally classified into two serologically distinct types, HPeV1 and -2, recent analyses of variants collected worldwide have revealed the existence 12 further types genetically by sequence comparisons complete genome sequences or capsid (VP1) gene. To investigate nature HPeV evolution, its population dynamics recombination breakpoints, this study generated 18 full-length genomic most commonly circulating genotypes, -3, over a time span 14 years from The Netherlands. By inclusion previously published sequences, 35 were analysed in total. Analysis contemporary strains those similar to prototype strain (Harris) showed that fall clusters much more divergent each other than observed within types. Future classification criteria for HPeVs may require modification accommodate occurrence with intermediate degrees diversity Recombination was frequently HPeV1, -4, -5 -6, but restricted HPeV3 strains. Favoured sites found flank region, non-structural P2. In contrast majority remained monophyletic across genome, possible reflection lower potentially emergence biological and/or epidemiological constraints limit opportunities co-infections potential partners.