Analysis of cassava brown streak viruses reveals the presence of distinct virus species causing cassava brown streak disease in East Africa

0301 basic medicine Manihot Genotype Sequence Homology, Amino Acid Molecular Sequence Data Potyvirus Genome, Viral Sequence Analysis, DNA Africa, Eastern Cross Reactions Antibodies, Viral 3. Good health 03 medical and health sciences Sequence Homology, Nucleic Acid Cluster Analysis RNA, Viral Capsid Proteins Phylogeny Plant Diseases
DOI: 10.1099/vir.0.014688-0 Publication Date: 2010-01-14T02:45:28Z
ABSTRACT
Cassava brown streak virus (CBSV) isolates were analysed from symptomatic cassava collected between 1997 and 2008 in the major cultivation regions of East Africa. An analysis complete RNA genomes seven Kenya, Tanzania, Mozambique, Uganda Malawi revealed a common genome structure, but clearly clustered two distinct clades. The first comprised Uganda, Malawi, north-western Tanzania CBSV described previously, shared 87 95% nucleotide sequence identity, whilst second included coastal Mozambique which only 70% identities with clade. When amino acid sequences viral proteins compared, as low 47% (Ham1) 59% (P1) clades found. antiserum obtained against capsid protein clade 1 isolate identified 43 kDa 45 2 isolates. Several cultivars susceptible to resistant those 1. differences observed both biological behaviour genomic indicate that disease Africa is caused by at least species. It suggested retain species name virus, be classified virus.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (23)
CITATIONS (169)