Sensitive and specific detection of sporadic Creutzfeldt–Jakob disease brain prion protein using real-time quaking-induced conversion

• DOI: 10.1099/vir.0.033365-0
• Publication Date: 2011-10-27T00:46:52Z
• Authors: Alexander H. Peden; Lynne I. McGuire; Nigel E. J. Apple...; Gary Mallinson; Jason M. Wilham; Christina D. Orrú; Byron Caughey; James W. Ironside; Richard S. Knight; Robert G. Will; Alison J. E. Green; Mark W. Head
• Keywords: Amyloid (mycology)

Real-time quaking-induced conversion (RT-QuIC) is an assay in which disease-associated prion protein (PrP) initiates a rapid conformational transition recombinant PrP (recPrP), resulting the formation of amyloid that can be monitored real time using dye thioflavin T. It therefore has potential advantages over analogous cell-free assays such as misfolding cyclic amplification (PMCA). The QuIC and related seeding have been developed largely rodent-passaged sheep scrapie strains. Given RT-QuIC for Creutzfeldt-Jakob disease (CJD) research human test development, this study characterized behaviour range CJD brain specimens with hamster recPrP assay. results showed rapid, sensitive specific form abnormal found most commonly occurring forms sporadic CJD. appeared to independent species-related sequence differences between methionine/valine polymorphism at codon 129 gene. However, same conditions substrate, was less efficient detecting characterizes variant brain. Comparison these those previously obtained PMCA suggested two seemingly similar differ important respects.